“Identities” reports the number of identical residues that are found aligned between the query and database sequence. The higher the score, the higher the similarity between query and database sequence. “Score” describes the quality of the alignment. “Length” reports the length of the database sequence. “Source” provides information on the source of the DNA, i.e.
Sequence alignment 4peaks code#
“DB:ID” gives the database identification code of the database entry/search result. the result with the best hit is assigned number 1). “Align.” reports the number which has been assigned to the search result according to the best alignment (i.e. Tool which can be used to reverse complement a nucleotide sequence.ĮNA result columns in NCBI BLAST+ (definitions) Forward and reverse sequencing reads for matK barcode.Reverse sequencing reads for matK barcode (please read note doc in folder).This sequence is identical to “TutorialExample”.Forward and reverse sequencing reads for rbcL barcode.Olea) to access the image.Ī folder containing all example data files (.seq and. Please enter the genus name of the identified plant (e.g. In case you would like to skip contig assembly, we have provided text files which contain the contig sequences of the respective examples.įollowing sequence preparation and search using the example data, the identity and an image of the plant in question can be viewed by clicking on “Example 1”, “Example 2”, etc. You may use them to assemble contigs and then search the ENA database for matches. abi (chromatogram information) files for. The example sequence data provide you with the opportunity to use barcode sequences without the need to carry out any experiments yourself. We also provide example sequence data which you can use in case you did not collect your own plant samples and wet-lab experimentation. On this page, we provide a summary of the bioinformatics tools to use for the analysis of your DNA sequencing data.
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